Lectin spatial immunolocalization during in vitro capacitation in Tursiops truncatus spermatozoa
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چکیده
منابع مشابه
Evaluation of in vitro capacitation of stallion spermatozoa.
The primary aim of this study was to establish a flow cytometric technique for determining the capacitation status of stallion spermatozoa. To this end, a flow cytometric technique that demonstrates changes in plasma membrane fluidity; namely, merocyanine 540 staining, was compared with the more conventional Ca(2+)-dependent fluorescence microscopic technique, chlortetracycline (CTC) staining, ...
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In this study, buffalo spermatozoa were in-vitro capacitated and acrosome reacted using modified Tyrode’s bicarbonatebuffered medium (sp-TALP media). Lipids were extracted from freshly ejaculated, in-vitro capacitated and acrosome reacted buffalo spermatozoa. The total lipid content of freshly ejaculated spermatozoa (171.5±2.90 μg/108 cells), capacitated spermatozoa (128.2±3.29 μg/108 cells) an...
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Flow cytometry was utilized to measure the influx of calcium into fresh and frozen-thawed equine and bovine spermatozoa that had been treated with either dilauroyl-phosphatidylcholine ( PC-12) or the calcium ionophore A23187 (A23187) to initiate sperm capacitation. Sperm suspensions were stained with the fluorescent calcium indicator, Fluo-3, to measure intracellular calcium concentrations and ...
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ژورنال
عنوان ژورنال: Animal Reproduction
سال: 2020
ISSN: 1984-3143
DOI: 10.21451/1984-3143-ar2019-0083